ICDDTM'18 Conference

Pre-conference Workshop

3rd December 2018 (Monday)  |  8.00 a.m. – 5.00 p.m.  

ICDDTM’18 is pleased to offer five pre-conference workshops in partnership with institutions and companies that are dealing with scientific researches. Workshops are not included in the main conference registration and will incur an additional fee.

1.   Workshop on Molecular Docking in Drug Discovery Research

Demonstrators: Prof. Dr. Kam Y. Zhang, Japan & Dr. Lam Kok Wai
Venue: Saintis Gemilang Room, Faculty of Science, Universiti Putra Malaysia
*Limited to 30 participants only

Introduction

This one-day workshop is exclusively tailored-made for beginners (students and researchers) with a diverse disciplines related to drug discovery or relevant applications. The main objective is to provide the participants an introduction to basic molecular docking with hands-on practice, thus improving the understanding of molecular mechanisms in specific biological activity. This computational technique may also help to conceive and evaluate potential molecules to become actual drugs. Last but not least, it would also increase the productivity by offering time-saving and cost-effective solutions in the development of new pharmaceutical products.

Content of the workshop

  • Theory On Molecular Docking And Computational Methods
  • Selection Of Target Receptor (Protein-Protein Interactions (Ppis) And Protein-Small Molecule Interactions)
  • Scoring Functions And Protein-Ligand Interaction
  • Hands-On Session On Performing Molecular Docking And Analysis Of The Docking Results

Who should attend?

  • The workshop is suitable for all personnel involved in natural product, organic synthesis, pharmaceutical, bio–medical, life sciences, structural biology, computational biology or those people who are intended to work with bioactive-small organic molecules. Participants are encouraged to bring their own laptop. Transportation will be provided to the Faculty of Science, Universiti Putra Malaysia from The Everly Hotel, Putrajaya.
2.0   CRISPR Gene Editing & Xmap Bead Coupling
         2.1   CRISPR Gene Editing 

Demonstrator: Dr. Lee Le Jie
Venue: The Everly Hotel, Putrajaya

Introduction

The development of efficient and reliable method to produce precise, targeted changes to the genome of living cells is a long-standing goal for biomedical research and eventually to be translated for therapeutic purpose in various disorders and diseases. CRISPR (Clustered Regularly Interspaced Short Palindormic Repeats) technology is a simple yet powerful tool for editing genomes. It allows researchers to easily alter DNA sequences and modify gene function. The protein Cas9 (CRISPR-associated) is an enzyme that acts like a pair of molecular scissors, capable of cutting strands of DNA. CRISPR genome editing allows scientists to quickly create cell and animal models, which, researchers can use to accelerate research into diseases such as cancer and mental illness. In addition, CRISPR is now being developed as a rapid diagnostic and therapeutic agent to correct dysfunctional genes.

This hands-on, laboratory-based workshop will provide an overview on this latest genome editing tool. The workshop will include lectures on basics of CRISPR, experiments together with discussion session which is fundamental for researchers who are interested to work on this cutting-edge technology.

Objective

To establish background knowledge and skills to design, construct and target genes to be modified by CRISPR.

Content of the workshop

Lecture

  • Designing specific vectors targeting gene of interest
  • Bioinformatics: designing gRNA and determining off-target
  • Efficient transfection of CRISPR/Cas
  • Downstream experiments crucial in determining efficacy and specificity of CRISPR

Practical

  • Choosing vector, designing gRNA, determining off-targets
  • Verifying CRISPR clones and transfection of CRISPR
  • Extracting gDNA to validate the efficiency of CRISPR 
         2.2   Xmap Bead Coupling

Demonstrator: Dr. Raymond Leong
Venue: The Everly Hotel, Putrajaya

Introduction

Biological assays have evolved from relatively large volume reactions to smaller volume faster with highly automated tests. Whether in a test tube rack, a microwell plate, or a micro-volume chip, these may all be considered as ‘arrays’ of assays. While solid phase technology such as microarrays (2-dimensional solid arrays) allow small-volume assaying of physically separated features, limitations such as slow kinetics, instability of immobilized protein and poor reproducibility may limit its broader application. Solution-phase multiplex assays remain highly desirable to laboratories as it reduces sample volume and other redundant consumables, faster and able to generate more data from the same amount of labour. Featuring a flexible open-architecture design, xMAP Technology can be configured to perform a wide variety of assays quickly, cost-effectively, and accurately. The development process for xMAP® multiplex assays is relatively simple, but does require a few unique considerations compared to monoplex assays. This workshop aims to expose a general workflow of xMAP assay development.

Objective

To establish background knowledge and skills to develop own custom immunoassay kit based on Luminex Xmap technology.

Content of the workshop

Lecture

  • Fundamental of antigen coupling
  • Coupling modification method
  • Coupling antigens to microsphere activity
  • Antigen coupling confirmation
  • Coupling guideline & FAQ

Practical

  • Xponent software exploration
  • Bead coupling procedure
3.   Pharmacometrics: Mathematical and Statistical Modelling for Drug Development and Personalised Medicine  

Demonstrators: Dr. Joseph Standing & Dr. Hadzliana Zainal
Venue: The Everly Hotel, Putrajaya

Introduction

Pharmacometrics studies the relationship between an administered dose of a medicine or other therapeutic, often an observed circulating concentration of the therapeutic, and a measured effect (efficacy and/or toxicity). Usually we are interested in how these evolve with time through repeated measures within individuals, and pooling information from multiple individuals to understand population effects. Pharmacometric models are used throughout drug development and are particularly crucial in deriving first-in-man dosing, designing and analysing Phase II dose-response experiments, in extrapolations to special populations, and increasingly in clinical model-based personalised/individualised medicine. The mathematical and statistical modelling approach of biologically plausible nonlinear models being fitted with mixed effects (multi-level) analysis has been extensively developed in clinical pharmacology. This technique is now being extended to vaccine development and understanding gene therapy effects. Participants will be required to bring a laptop with R (version 3.4 or higher), Rstudio, and the nlmixr package (including all dependant packages) installed.

Objective

To establish background knowledge and skills to design, conduct and analyse data from pharmacometric experiments.

Content of the workshop

Lecture

  • Biological basis of pharmacokinetic and pharmacodynamic models
  • Mathematical and statistical underpinning of nonlinear mixed effects modelling
  • Designing and conducting pharmacometric studies
  • Model evaluation and covariate analysis and applications

Practical

  • Descriptive analyses of nonlinear data
  • Fitting a nonlinear mixed effects model using nlmixr
  • Model evaluation and covariate analysis
4.   Mapping the Tissue Microenvironment through Quantitative Pathology Solutions  

Demonstrator: Preston Teng
Venue: The Everly Hotel, Putrajaya

Morning Session:

  • Introduction of Quantitative Pathology Solution
  • Product review and software features (what do we have and how can we help)

Afternoon Session:

  • Hands on session on Vectra 3 Imaging system on demo slides.
  • Participant can discuss with us if they would like to bring their own slide for viewing.
5.  Biomarker Discovery and Translational Research: Challenges and Solutions in Drug Discovery

Demonstrator: Dr. Erhan Simsek
Venue: The Everly Hotel, Putrajaya

Introduction

Metabolic pathway mapping and visualization allows scientists to focus research in active biological areas. Mapping the results of a traditional discovery metabolomics experiment onto pathways can more rapidly narrow the list of metabolite targets. Because of the interconnectivity of metabolites, proteins and genes, any omic experiment can be the starting point for the next experiment using pathways as a framework to interpret the results and propose further experiments. This workshop will demonstrate both a metabolomic discovery-based workflow and proteomic-directed targeted workflow. These approaches can be incorporated into biomarker discovery as part of a drug discovery pipeline and through a program of translational research.

Objective

To provide knowledge of the approaches that can be used in a drug discovery and translational research program and to provide information on the tools and supporting software for data interpretation.

Content of the workshop

Lecture

  • Multi-omics in biomarker discovery
  • Pathway mapping to visualize metabolite, protein and gene information
  • Targeted protein analysis to validate metabolite-discovery experiments

​Practical

  • Mass Profiler Professional and Pathway Architect software demonstration